Unpublished video clips
- Interphase microtubule dynamics in Xenopus S3 cell, imaged with MizarTILT lightsheet microscopy (1 sec intervals, in collaboration with Paul Maddox, Univ of North Carolina)
[video] - Anaphase microtubule dynamics in Xenopus S3 cell, imaged with MizarTILT lightsheet microscopy (10 sec intervals, in collaboration with Paul Maddox, Univ of North Carolina)
[video] - Normal cell division in cultured Xenopus S3 cell (phase contrast)
[low resolution] [high resolution] - Normal cell division in cultured Xenopus S3 cell (phase contrast and GFP-tubulin)
[video] - Cell division with late aligning chromosomes, our most popular video (phase contrast)
[low resolution] [high resolution] - Human epithelial cell mitosis
[low resolution] - Glenn Wolkenfeld’s Mitosis Rap (with a Gorbsky lab video on the chorus!)
[music video]
Publications with video clips
- Sapkota H, Wasiak E, Daum JR, Gorbsky GJ.
Multiple determinants and consequences of cohesion fatigue in mammalian cells.
Mol Biol Cell. 2018 Aug 1;29(15):1811-1824. doi: 10.1091/mbc.E18-05-0315. Epub 2018 May 30. [Videos] - Tipton AR, Wren JD, Daum JR, Siefert JC, Gorbsky GJ.
GTSE1 regulates spindle microtubule dynamics to control Aurora B kinase and Kif4A chromokinesin on chromosome arms.
J Cell Biol. 2017 Oct 2;216(10):3117-3132. doi: 10.1083/jcb.201610012. Epub 2017 Aug 18. [Videos] - Sivakumar S, Daum JR, Tipton AR, Rankin S, Gorbsky GJ.
The spindle and kinetochore-associated (Ska) complex enhances binding of the anaphase-promoting complex/cyclosome (APC/C) to chromosomes and promotes mitotic exit.
Mol Biol Cell. 2014 Mar;25(5):594-605. Epub 2014 Jan 8. [Videos] - Wang F, Ulyanova NP, Daum JR, Patnaik D, Kateneva AV, Gorbsky GJ, Higgins JM.
Haspin inhibitors reveal centromeric functions of Aurora B in chromosome segregation.
J Cell Biol. 2012 Oct 15;199(2):251-68. [Videos] - Daum JR, Potapova TA, Sivakumar S, Daniel JJ, Flynn JN, Rankin S, Gorbsky GJ.
Cohesion fatigue induces chromatid separation in cells delayed at metaphase.
Curr Biol. 2011 Jun 21;21(12):1018-24. Epub 2011 Jun 9. [Videos] - Potapova TA, Sivakumar S, Flynn JN, Li R, Gorbsky GJ.
Mitotic progression becomes irreversible in prometaphase and collapses when Wee1 and Cdc25 are inhibited.
Mol Biol Cell. 2011 Apr;22(8):1191-206. Epub 2011 Feb 16. [Videos] - Wang F, Dai J, Daum JR, Niedzialkowska E, Banerjee B, Stukenberg PT, Gorbsky GJ, Higgins JM.
Histone H3 Thr-3 phosphorylation by Haspin positions Aurora B at centromeres in mitosis.
Science. 2010 Oct 8;330(6001):231-5. Epub 2010 Aug 12. [Videos] - Daum JR, Wren JD, Daniel JJ, Sivakumar S, McAvoy JN, Potapova TA, Gorbsky GJ.
Ska3 Is Required for Spindle Checkpoint Silencing and the Maintenance of Chromosome Cohesion in Mitosis.
Curr Biol. 2009 Jul 29. [Videos] - Salmela AL, Pouwels J, Varis A, Kukkonen AM, Toivonen P, Halonen PK, Perälä M, Kallioniemi O, Gorbsky GJ, Kallio MJ.
Dietary flavonoid fisetin induces a forced exit from mitosis by targeting the mitotic spindle checkpoint.
Carcinogenesis. 2009 Jun;30(6):1032-40. Epub 2009 Apr 24. [Videos] - Knowlton AL, Vorozhko VV, Lan W, Gorbsky GJ, Stukenberg PT.
ICIS and Aurora B Coregulate the Microtubule Depolymerase Kif2a.
Curr Biol. 2009 Mar 25. [Videos] - DeMoe JH, Santaguida S, Daum JR, Musacchio A, Gorbsky GJ.
A high throughput, whole cell screen for small molecule inhibitors of the mitotic spindle checkpoint identifies OM137, a novel Aurora kinase inhibitor.
Cancer Res. 2009 Feb 15;69(4):1509-16. Epub 2009 Feb 3. [Videos] - Potapova TA, Daum JR, Byrd KS, Gorbsky GJ.
Fine tuning the cell cycle: activation of the Cdk1 inhibitory phosphorylation pathway during mitotic exit.
Mol Biol Cell. 2009 Mar;20(6):1737-48. Epub 2009 Jan 21. [Videos] - Hu L, Plafker K, Vorozhko V, Zuna RE, Hanigan MH, Gorbsky GJ, Plafker SM, Angeletti PC, Ceresa BP.
Human papillomavirus 16 E5 induces bi-nucleated cell formation by cell-cell fusion.
Virology. 2009 Feb 5;384(1):125-34. Epub 2008 Nov 28. [Videos] - Vorozhko VV, Emanuele MJ, Kallio MJ, Stukenberg PT, Gorbsky GJ.
Multiple mechanisms of chromosome movement in vertebrate cells mediated through the Ndc80 complex and dynein/dynactin.
Chromosoma. 2008 Apr;117(2):169-79. Epub 2007 Dec 4. [Videos] - Eyster CA, Duggins QS, Gorbsky GJ, Olson AL.
Microtubule network is required for insulin signaling through activation of Akt/protein kinase B: Evidence that insulin stimulates vesicle docking/fusion but not intracellular mobility.
J Biol Chem. 2006 Oct 26; [Epub ahead of print] [Videos] - Potapova TA, Daum JR, Pittman BD, Hudson JR, Jones TN, Satinover DL, Stukenberg PT, Gorbsky GJ.
The reversibility of mitotic exit in vertebrate cells.
Nature. 2006 Apr 13;440(7086):954-8. [Videos] - Beardmore VA, Ahonen LJ, Gorbsky GJ, Kallio MJ.
Survivin dynamics increases at centromeres during G2/M phase transition and is regulated by microtubule-attachment and Aurora B kinase activity.
J Cell Sci. 2004 Aug 15;117(Pt 18):4033-42. Epub 2004 Jul 27. [Videos] - McCleland ML, Kallio MJ, Barrett-Wilt GA, Kestner CA, Shabanowitz J, Hunt DF, Gorbsky GJ, Stukenberg PT.
The vertebrate Ndc80 complex contains Spc24 and Spc25 homologs, which are required to establish and maintain kinetochore-microtubule attachment.
Curr Biol. 2004 Jan 20;14(2):131-7. [Videos] - McCleland ML, Gardner RD, Kallio MJ, Daum JR, Gorbsky GJ, Burke DJ,
Stukenberg PT.
The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity.
Genes Dev. 2003 Jan 1;17(1):101-14. [Videos] - Kallio MJ, Beardmore VA, Weinstein J, Gorbsky GJ.
Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells.
J Cell Biol. 2002 Sep 2;158(5):841-7. Epub 2002 Aug 26. [Videos] - Tavormina PA, Come MG, Hudson JR, Mo YY, Beck WT, Gorbsky GJ.
Rapid exchange of mammalian topoisomerase II alpha at kinetochores and chromosome arms in mitosis.
J Cell Biol. 2002 Jul 8;158(1):23-9. Epub 2002 Jul 8. [Videos]